Journal: World Journal of Clinical Oncology
Article Title: Parthenolide enhances the metronomic chemotherapy effect of cyclophosphamide in lung cancer by inhibiting the NF-kB signaling pathway
doi: 10.5306/wjco.v15.i7.895
Figure Lengend Snippet: Parthenolide alleviated cyclophosphamide-induced NF-kB activation. A: NF-κB immunohistochemistry staining as well as comparison of the percentage of total and nuclei NF-κB positive cells in transplanted tumors of mice in different treatment groups. Magnification: 200 ×; B: P-NF-κB and NF-κB protein immunoblotting as well as gray analysis. Full undamaged Gels and Blots images are shown in Supplementary Figure 2; C: Analysis of NF-κB P65 transcriptional activity. n = 10, a P < 0.05; c P < 0.001; NS: Not significant. PTL: Parthenolide; CTX: Cyclophosphamide.
Article Snippet: Transcription factor activity for NF-kB was carried out with a commercial NF-κB p65 transcription factor activity assay kit (RayBiotech Inc., United States) which coded plate with NF-κB binding DNA sequence that specifically captured the active NF-κB p65 contained in whole cell lysate or nuclear extracts.
Techniques: Activation Assay, Immunohistochemistry, Staining, Comparison, Western Blot, Activity Assay